Generator

Part:BBa_K737012

Designed by: Tianhe Wang   Group: iGEM12_OUC-China   (2012-09-18)

gvpC-GFP fusion protein UH with medium constitutive promoter

This part composed of fusion gene between gvpC-20 and GFP mut 3.1 by using rigidity hinge which Length can achieve 41 Å and Fad-Fad Angle is 123 °a The fusion protein is used to detect the expression of gvpC within the cell volume.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1265
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal SpeI site found at 1265
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1265
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1265
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1187


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Categories
Parameters
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